用锁定的核酸间隙聚体敲除核定位增强子RNA和长NCRNA

The human genome is widely transcribed outside of protein-coding genes,producing thousands of noncoding RNAs from different subfamilies including enhancer RNAs.确定单个基因作用的功能性研究具有挑战性,非编码RNA似乎比mRNAs更难被击倒。可能阻碍进展的一个因素是大多数非编码RNA被认为位于核内,where the efficiency of traditional RNA interference techniques is debatable.Here researchers from theUniversity of Bathpresent an alternative RNA interference technique utilizing Locked Nucleic Acids,无论细胞内位置如何,都能有效地抑制非编码RNA。

长非编码RNA的一些功能概述bepaly买球

LNCRNA

bepaly买球lncRNAs are involved in gene regulation through a variety of mechanisms.lncrna本身的转录过程可以作为转录的标记,产生的lncrna可以在顺式和反式的转录调节或染色质修饰(通常通过DNA和蛋白质相互作用)中发挥作用。bepaly买球lncrnas能与互补的RNA结合并影响RNA的加工。turnover or localization.lncrna与蛋白质的相互作用可以影响蛋白质的功能和定位,并促进核糖蛋白复合物的形成。Some bepaly买球lncRNAs are actually precursors for smaller regulatory RNAs such as microRNAs or piwi RNAs.图修改自威鲁斯等.Genes Dev.2009。23:1494-154。PMID: 19571179.

鲁克斯,Lindsay MAHeward JA.(2016) 用锁定的核酸间隙聚体敲除核定位增强子RNA和长NCRNA. Methods Mol Biol1468:11-8.[ abstract]

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